Amanita pseudovaginata from Pakistan

Amanita pseudovaginata of Amanita subgenus Amanita sect. Vaginatae was found associated with Quercus spp. forests during a survey of macrofungi from oaks forests of Pakistan. The fruiting body was characterized morphoanatomically as well as by molecular analysis. The identification of the fungal symbiont as Amanita pseudovaginata was confirmed by Internal Transcribed Spacer Region (ITS) sequences. Sporocarps were matched with published data available from Russia and China. Phylogenetic analyses and morphological descriptions are provided. This represents the first report of this species in Pakistan.