ANTIBACTERIAL ACTIVITY OF NIGELLA SATIVA AND PIPER NIGRUM

The aim of the present research was to find out the antibacterial activity of Nigella sativa and Piper nigrum against the 2 gram positive and 3 gram negative bacteria. The present research was carried out in the Microbiology Research Laboratory Hazara University Mansehra Khyber Pukhtunkhwa Pakistan during 17 January to 22 April 2011. In the present research Disc diffusion and Agar well plate method were used. The results of the Ager well plate method as more clear as compare to Disc diffusion method. The cold water, hot water and methanolic extract of the Nigella sativa have high activity against Escherichia coli 23mm, 22mm and 23mm respectively. The extracts of the said plant have shown low activity 18mm against Pseudomonas aeruginosa and Enterococcus faecalis. The methanolic extract of the Nigella sativa have no activity against Staphylococcus aureus. The same extracts were also obtained from Piper nigrum. The cold water extract give good result 23mm against Escherichia coli while less effective against Pseudomonas aeruginosa 15mm. The cold water and methanolic extract have high activity against Salmonella typhae, Escherichia coli and low activity against Pseudomonas aeruginosa and Enterococcus faecalis respectively. Similarly the methanolic extract of Piper nigrum has shown no activity against Staphylococcus aureus. From the present research it was concluded that all the extract of Nigella sativa and Piper nigrum have high activity against gram negative bacteria as compare to gram positive bacteria.