Influence of Phyllanthus Amarus on Biochemical and Haematological Status in Rats with Ibuprofen-Induced Nephrotoxicity

This present study was designed to evaluate the influence of 70% alcohol extract of Phyllanthus amarus leaf (AEPA) on the biochemical and haematological status in rats with ibuprofen-induced nephrotoxicity. Twenty-four albino Wistar rats (150-190 g) of both sexes were randomly grouped into six of four rats per group. Group I served as the control and was administered distilled water. Groups II-VI were induced by oral gavage with 200 mg/kg b.wt. of ibuprofen single dose. On the third day of the experiment, group III was treated with 0.097 mg/kg b.wt. of termisartan drug while groups IV, V and VI were treated with 50, 100 and 200 mg/kg b.wt./day of AEPA respectively for 28 days. Then, biochemical and haematological parameters were measured while histological study was carried out on kidney sample. No significant (p>0.05) change in body weight of animals throughout the experimental period. Treatment of rats with 50-200 mg/kg b.wt of P. amarus leaf extract and termisartan drug significantly (p<0.05) reduced the levels of creatinine, AST, ALP. RBC, HB, HCT and PLT and significantly (p<0.05) increased ALT, total protein and albumin levels, monocyte and neutrophil counts compared to the ibuprofen-induced rats group. No significant (p>0.05) alterations were observed in WBC, MCV, MCH, MCHC and lymphocytes. The induced morphological architecture alterations in the kidney by ibuprofen were reversed with increased doses of P. amarus. Therefore, AEPA has selective ameliorative influence on the biochemical and haematological parameters in ibuprofen-induced nephrotoxic rats.