Effect of alpha lipoic acid and trehalose on ram semen cryopreservation

In this study, it was aimed to investigate the effects on spermatozoon motility, plasma membrane and acrosome integrity (PMAI), mitochondrial membrane potential and mitochondrial reactive oxygen species after freezing and thawing by adding different doses of A Lipoic acid (ALA) and Trehalose to ram semen extender. Semen was collected from 10 Pirlak rams using artificial vagina, divided into three equal parts, extended with extender containing A lipoic acid (1 mM), Trehalose (100 mM) and no additives (control) to contain 5x108/ ml spermatozoa. Sperm was equilibrated about 2 hours at + 4 ° C, frozen in liquid nitrogen vapor (-120 ° C), thawed in 30 seconds at 37 ° C. Thawed sperm sample were evaluated. Subjective motility evaluated by phase contrast microscope with heating table at 37 °C, PMAI, mitochondrial membrane potential and mitochondrial reactive oxygen species were evaluated by flow cytometer. While the lowest motility was detected in the control group, the highest plasma membrane integrity was found in the Trehalose group (P <0.05). While the lowest Hmmp and the highest Lmmp value were detected in the control group, the lowest MitoSOX+ was detected in Trehalose group. It has been determined that ALA and Trehalose improve spermatological parameters in Pirlak ram semen after freezing and thawing procedure on non-breeding season.