First report on black spot disease of Phyllanthus emblica L. fruits caused by Thielaviopsis paradoxa in Bangladesh
Journal: International Journal of Agricultural Research, Innovation and Technology (IJARIT) (Vol.10, No. 2)Publication Date: 2020-12-31
Authors : M.M. Sikder Ahmmed A. Sultana; N. Alam;
Page : 38-46
Keywords : Amla; Fruit spot disease; Fungal biology; Thielaviopsis paradoxa; Molecular characterization;
Abstract
Fruit spot disease of Phyllanthus emblica L. is one of theproblems, which reduce the quality of the fruits at pre-harvest period. Fungal pathogen was isolated using tissue planting methods during November 2016 to December 2017. The fungus, Thielaviopsis paradoxa was identified using both morphological and molecular characterization based on internal transcribe spacer (ITS) region of ribosomal DNA (rDNA). Mycelial growth of the isolated fungus was evaluated on six different fungal culture media viz, potato sucrose agar (PSA), Richard agar (RA), carrot agar (CA), potato dextrose agar (PDA), honey peptone agar (HPA) and Hansen's agar (HA) in which RA and HPA media provided the utmost growth. The optimum temperature of the fungus was recorded at 25 to 35ºC. Alternate cycle of 12h/12h light dark and neutral to basic pH was preferred by the studied fungus. Aqueous crude extracts of three plants (garlic, black cumin, and turmeric) were evaluated against the isolated fungus in which the highest inhibition was recorded due to garlic extract. Two food preservatives (sodium benzoate and vinegar) were also tested in which sodium benzoate (100 mM) was most efficient for the inhibition of T. paradoxa. Therefore, garlic and food preservative-sodium benzoate could be used to control this fungal growth associated with amla fruits. To the best of our knowledge, occurrence of T. paradoxa on amla fruits is a new record in Bangladesh.
Other Latest Articles
- Evaluation of black cumin genotypes for yield and yield related parameters in bale mid altitude, southeastern Ethiopia
- Effect of Integrated Nutrient Management on Growth, Yield Attributes and Yield of Wheat
- Various inputs applied for growing sweet potato in the tidal swamp area of south Borneo of Indonesia
- Comparative analysis of chickpea with boro rice in drought-prone areas of Bangladesh
- Application of probiotics and prebiotics for promoting growth of Tiger shrimp (Penaeus monodon): an approach to eco-friendly shrimp aquaculture
Last modified: 2021-02-05 22:56:28