A STUDY OF THE CORRELATION BETWEEN HISTOPATHOLOGICAL EXAMINATION OF THEHEPATOBILIARY TISSUE WITH SERUM ALANINE AMINOTRANSFERASE AND ALKALINE PHOSPHATASE

Background: Several biochemical tests are useful in the evaluation and management of patients with hepatic dysfunction.These tests can be used to detect the presence of liver disease, distinguish among different types of liver disorders, gauge the extent of known liver damage, and follow the response to treatment.ALT reflects damage to hepatocytes and it is found primarily in liver in low concentrations and get released into the blood in greater amount when there is damage to liver cell membrane resulting in increased permeability.The activity of ALP in a pathological condition is usually elevated in cholestasis.Non-pathologically it is also elevated in children and adolescents undergoing rapid bone growth and in late pregnancy.The main objective of this study was to correlate these serum biomarkers with the histopathological reports of the resected gallbladder specimen. Methodology: This study was conducted prospectively on 493 patients in a tertiary care hospital in eastern Uttar Pradesh (India).In year 2019, from January to December, patients admitted for cholecystectomy in surgery ward of this hospital were included in this study.After surgery, about 3-4 ml of the blood sample was obtained and analyzed in the biochemistry laboratory after proper control and calibration, meeting the quality standard of the laboratory.Results thus obtained were correlated with the histopathological report of the gallbladder specimen provided by the pathology department of the institute. Results: In our study, out of 493 patients it was observed that 70 (14.2%) patients were males with mean age of 39.6 years and 423 (85.8%) patients were females with mean age of 37.6 years.Chronic cholecystitis with cholelithiasis was reported in 284 (57.6%) patients with mean values of serum ALT as 79.9 IU/L and serum ALP as 263.9 IU/L.Chronic cholecystitis was reported in 79 (16%) patients with mean values of serum ALT as 59.1 IU/L and serum ALP as 228.1 IU/L. Conclusion: From our study, we concluded that in patients of chronic cholecystitis with cholelithiasis, observed values of serum ALT and serum ALP were much higher than in chronic cholecystitis.