Development and Validation of a RP-HPLC Method for Simultaneous Quantitation of Resveratrol and Curcumin: Application to Nanolipid Gel Formulation

A new RP-HPLC method for quantitation of resveratrol and curcumin was developed and validated as per ICH Q2 (R1) guideline. Resveratrol and Curcumin shows synergistic effect. Both the drugs either stand alone and in combination have been utilized in the development of many novel formulations. However, no report is available for their identification and quantitation in combined dosage form. This new method was developed for simultaneous quantitation of resveratrol and Curcumin in a Nanolipid gel formulation.The chromatographic conditions were optimized using Hiber C-18 column, (4.6 mm × 250 mm, 5 μm) with acetonitrile: 0.01 M phosphate buffer pH adjusted to 4.0 ± 0.05 using 1 % v/v o-phosphoric acid (60:40, v/v) as mobile phase. The flow rate was 1 ml/min and detection was performed at 345 nm. The retention times of resveratrol and curcumin were found to be 3.13 min and 6.59 min, respectively. Linearity for both the drugs was established in the range of 10-60 µg/ml. The percentage recoveries of resveratrol and curcumin were found to be in the range of 99.5-101.75 and 99.01-101.05, respectively. The % RSD values for intraday and interday precision were found to be 0.10 and 0.8 for resveratrol and 0.30 and 0.58 for curcumin. LOD and LOQ for resveratrol and curcumin were found to be 0.11 & 0.29 µg/ml and 0.34 & 0.90 µg/ml, respectively. Mean % assay ± S.D. for resveratrol and curcumin in Nanolipid gel formulation using the proposed method were found to be 101.67 ± 0.02 and 98.66