The Application of Modern Biotechnology in Protein Interaction Research-A Review
Journal: Journal of Agriculture and Crops (Vol.7, No. 2)Publication Date: 2021-02-02
Authors : Bo Peng; Xia-Yu Tian; Zi-Yue Liu; Juan Peng; Meng-Yang Zheng; Xiao-Hua Song; Ya-Qin Huang; Xin-Xiang A; Yan-Ming Zhang; Fang Yang; Hui-Long Li; Yan-Yang Sun; Rui-Hua Pang; Wei Zhou; Quan-Xiu Wang; Hongyu Yuan;
Page : 39-47
Keywords : Protein interaction; Biotechnology; Theory; Application; New progress.;
Abstract
Intermolecular interaction is the material basis for cells to achieve their functions, and protein-protein interaction is an important approach to illuminate the regulation network of biological molecules and has important theoretical significance and potential application value for revealing the activity law of life in nature. This paper mainly summarizes and analyzes the new advances and applications of modern biotechnologies in the study of protein-protein interactions, including local surface plasmon resonance (LSPR), yeast two-hybrid, GST-Pull-down, bimolecular fluorescence complementation, and coimmunoprecipitation. At the same time, the principles of different research methods for protein-protein interaction and their other applications in the field of life sciences are also discussed, all of these will provide a reference value for the analysis of protein-protein interaction and the molecular regulation mechanism of biomacromolecules.
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Last modified: 2021-03-03 19:26:48