Isolation, Identification and Characterization of Thermo-tolerant Bacteria from Hot

To investigate the microbial community in the hot spring of Krabi, Thailand and if they can degrade some halogenated compounds. A thermophilic bacterium (YKH) was isolated from the Krabi hot spring in Thailand. The isolate had entire and slimy colonies while the cells were small rods and gram-positive. The growth profile of YKH was measured at 60 OC in different concentrations of 2,2 DCP (5 mM, 10 mM, 15 mM, 20 mM and 25 mM). The full length sequence with around 1220 base pairs of the sample YKH was obtained. Full sequence of YKH was blasted via National Center of Biotechnology Information (NCBI) to be compared with other known bacteria species and the similarities with other species were obtained. BLAST search result of partial 16S rRNA sequence obtained showed that YKH has close matches with Bacillus sp. with 100% and 99% similarities respectively. Result from BLAST suggested that strain YKH belongs to the genus Bacillus sp..The identification and the characterization are depending on the 16S rRNA and the morphological, biochemical tests respectively. The results of the BLAST search results represented by MEGA4 (phylogenetic tree). According to previous researches up to date, the study of degradation of halogenated compounds by thermophlic bacteria is very limited. This study focuses on the degradation of these compounds by thermo-tolerant bacteria. So, the results of this study allow us to make a library of the microorganisms which are able to degrade 2, 2-dichloropropionic and classify them based on genus and species analysis. These bacteria strains might be important for future study on the enzyme dehalogenase that can act at high temperature.