The Study Effects of the Hand washing on Hands Bacterial Flora in Operating Room

Introduction: Hand washing and disinfection procedures before surgery is associated with reduced of the bacterial flora. But the decline is unclear. The aim of this study was to determine the effect of disinfectant solutions used in the operating room on the bacterial flora of the population. Methods: In a cross-sectional study in two stages, and each time the effect of disinfectant solutions on the physicians and staff hands washing were randomly performed. Experimental design was with four phases, in the first and second stage, before hands washing and fingers tip touch and transfer washing solutions onto the culture media were as sampling method. Step 3 and 4 was repeated as before. Inoculated media were incubated for 24 hours. The results have grouped and analyzed using descriptive statistics were carried out. Results: The findings of this study consisted of aerobic bacteria. The anaerobic bacteria do not include. The results indicate that isolated bacterial populations were highly diverse and more than 25 species of aerobic bacteria were detected. The results indicate that the hand flora in wet condition was more than 10 times. The most common organisms isolated were Staphylococcal SPP. An amazing finding was that the isolate Escherichia coli and Acinetobacter SPP. 4 out of the 25 bacterial isolate had hemolytic and coagulase production. Based on our results, none of the disinfectants solutions used did not able completely remove the natural hands bacterial flora. Conclusion: Hand washing for removal of hands bacterial flora is not sufficient for 3 minutes. Drying hands could be decline bacterial flora associated with the 90% to 95% in all cases, but did not 100%. Therefore, it seems that other procedure may be necessary for complete control of the hands bacterial flora population.