BioSynthesis of Silver Nanoparticles Using Putrajiva Roxyburghii Wall Leaves extract and their Anti Bacterial Activities

The synthesis of nanoparticles from plant source has to be prove to be effective and alternate method for the noble production of nanoparticles .A number of synthesis technique have been developed including chemical method ,physical method and biosynthetic method. In this research synthesis of silver nanoparticles using putranjiva roxyburghii wall leaves has been investigated .we successfully synthesized uniformly dispersed silver nanoparticles with the size and shape in the range of 3 to 20nm with an average size of 6 nm .The characterization of nanoparticles was done by using UV-VIS spectrophotometer and fluorescent spectrometer.the resulting formation of silver nanoparticles are characterized by using Transmission electron microscopy(TEM) .The TEM study shows the formation of silver nanoparticles .The antibacterial activity of these nanoparticles was studied against E.coli and staphylococcus aurenus .The bacterial property was analysed by measuring the growth curve of bacteria and 50ug/ml concentration of silver nanoparticles was found to be effective antibacterial.