Microbial Synthesized Silver Nanoparticles for Decolorization and Biodegradation of Azo Dye Compound

Biological approach for silver nanoparticle synthesis using microorganisms like Bacteria, Fungi, algae and plants have received profound interest because of their potential to synthesize nanoparticles of various size, shape and morphology. The present research work focus on bacterial synthesis of silver nanoparticles their efficiency forbioremediation of Congo red dye. Twenty one types of different bacterial species have been isolatedfrom the different industrial waste effluent and bacterial consortium was prepared. Potential silver resistant bacteria were isolated from bacterial consortium using scale up method and identified as Bacillus pumillus by Gram staining, biochemical tests and genome sequencing method. After that Silver nanoparticles were synthesized using potential silver resistant bacteria. Silver nitrate and silver sulfate were used as precursor at different concentration for silver nanoparticle synthesis. Color change from colorless to brownwas observed synthesis of silver nanoparticles was further confirmed by UV Visible spectroscopy. The silver nanoparticles formation was (5-93 nm in size) also confirmed by Transmission electron microscopy, Particle size analyzer, Dynamic light scattering depending on the concentration of silver precursor.In this research, we have shown for the first time the use of Bacillus pumillis for synthesis of silver nanoparticles. Dye decolorization and biodegradation was studiedusing silver nanoparticles, silver resistant bacteria and silver resistant bacteria influced by silver nanoparticles separately. Nano based bioremediation was found 13 % efficient than the microbial remediation. Thus the developed nano-bioremediation technology is biocompatible, simple and reliable method and can be applied to decolorize dye as well as antimicrobial agent.