Intestinal Bacterial Composition and Characteristics of Wintering Mandarin Duck (Aix Galericulata) in Shiqian Mandarin Duck Lake National Wetland Park, Guizhou Province

Intestinal bacteria are closely related to host's nutrition, metabolism and immunity. Therefore, understanding its composition is the basis for understanding health status of the host. In this study, using high-throughput sequencing of V3-V4 variable region of 16S rRNA, 14 fresh feces of Mandarin Ducks form Shiqian Mandarin Duck Lake National Wetland Park, Guizhou Province were examined to analyze composition and characteristics of intestinal bacteria. Results are as follows: 1107760 valid sequences and 74837 OTU are obtained by sequencing. 244 genera, 130 families, 65 orders, 40 classes and 22 phyla of bacteria are detected, among which 166 genera, 107 families, 60 orders, 40 classes and 22 phyla are identified. Firmicutes (45.72%), Proteobacteria (23.71%), Fusobacteria (14.05%) and Bacteroidetes (10.21%) are dominant phyla (93.69%). Fusobacterium (13.95%), Lactobacillus (6.74%), Pseudomonas (4.72%), Akkermansia (4.23%) and Bacteroides (3.98%) are dominant genera. Fusobacterium and Lactobacillus are common in all samples. Alpha diversity indices are significant differences in bacterial groups of samples, which might be related to daily food intake of Mandarin Ducks. However, the specific differences or influencing mechanism needed to be studied on the relationship between host's diet and intestinal bacteria. This study enriches micro ecology contents of Mandarin Duck, and has certain reference value for evaluating the survival and health conditions of Mandarin Ducks.