Study on Biochemical Profile of Improved Cattle at Various Stage of Lactation in Rupandehi District of Nepal

Objective: To determine the reference value of biochemical parameters of improved cattle of Rupandehi district of Nepal. Methodology: The sampling was done from the various commercial farm of Rupandehi district of Nepal. A total of 53 improved cattle (apparently healthy) were selected from farmers. It was cross sectional purposive sampling. Those cattle were divided into various predefined groups: 1. Dry periods (last 3 weeks of pregnancy) 2. Early lactating (calving to 3 weeks) 3. Mid lactating (2-4 months) and 4. Late lactation (after 4months). The serum analysis was done by using manually operated refractometer for total protein and Calorimeter with the available reagent in the market for all other parameter. Statistical analysis was done by using SPSS-20 using one way ANOVA. The data was presented as mean±standard error (SE). Result: Total protein was significantly different (P<0.05) at various stage of lactation with highest in III stage, globulin was significantly different (P<0.05) at various stage of lactation with highest value in II stage and BUN was also significantly different (P<0.05) at various stage of lactation with highest value in III stage. The parameters like albumin, SGPT, SGOT, ALP, Creatinine, Calcium and Phosphorous were non- significantly different (P>0.05) among various stage of lactation. Conclusion: This result demonstrated the reference value of various biochemical parameters of improved cattle at various stage of lactation of Rupandehi district which could be used by veterinarian as a base for Evidence based Veterinary Medicine.