Determination of the Presence of Pesticides Insecticide Residual Concentrations in Serum Blood Samples of Albino Rats Exposed to Mosquito Net Treated Insecticide over a Long Period as a Case Study

The study was designed to experimentally investigate the presence of permethrin and cypermethrin insecticide residue concentrations in serum samples of albino rats. 60 Albino rats were used in this research which were equally divided into three groups, with the first containing Rats placed under no mosquito net as control, the second containing Rats placed under commercially insecticide treated Mosquito net, and the third containing rats placed under experimentally formulated treated insecticide Mosquito net. The presence of the insecticide concentration in Blood serum samples of the Rats were determined after carefully extracting the serum by solvent extraction technique, and analyzing the extracts by Gas Chromatographic method. Permethrin insecticide and cypermethrin insecticide were detected in the serum samples of the albino Rats. ANOVA results of F 2,57 =9.1,P=0.000 indicates significant difference in the concentration level of the insecticide in the three groups of the Rats. The Post hoc test indicates that, those Rats housed under experimentally formulated mosquito net, Mean=0.8,SD=0.02 , and those placed under commercially insecticide treated Mosquito net, Mean=0.5,SD=0.001 , experienced higher level of concentration of insecticide as compared to controls, Mean=0.00,SD=0.00 those in Group A . The Mean ± S.D for permethrin and cypermethrin insecticides were significantly different from that of controls. 0.9±0.011mg L of Permethrin was detected in samples of week two and three of the commercially insecticide treated Mosquito net and 0.32.±0.01mg L in week one of the experimentally formulated treated insecticide Mosquito net, respectively as indicated in Table 1, while cypermethrin has a highest value of 0.1±0.082mg L in week two, six, and seven of the commercially insecticide treated Mosquito net as well as 0.9±0.082mg L in the experimentally formulated treated insecticide Mosquito net respectively. The values recorded for both insecticides used in this work are within the WHO recommended permissible limit of 0.05mg kg 2.0mg kg. Muktar Haruna Dunari | Hussaini Abubakar | Haruna Abubakar Danyaya | Balarabe Sarki Sagagi "Determination of the Presence of Pesticides/Insecticide Residual Concentrations in Serum Blood Samples of Albino Rats Exposed to Mosquito Net Treated Insecticide over a Long Period as a Case Study" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38484.pdf Paper Url: https://www.ijtsrd.com/chemistry/chromatography/38484/determination-of-the-presence-of-pesticidesinsecticide-residual-concentrations-in-serum-blood-samples-of-albino-rats-exposed-to-mosquito-net-treated-insecticide-over-a-long-period-as-a-case-study/muktar-haruna-dunari