OPTIMIZATION OF EXPERIMENTAL VARIABLES FOR THE PRODUCTION OF - AMYLASE BY ASPERGILLUS ORYZAE USING RICE BRAN

This investigation is made to optimize the experimental parameters for the production of - amylase by Aspergillus oryzae MTCC-8624 on using rice bran as a cheaper substrate. pH, temperature, fermentation time, inoculum concentration and substrate using a Box–Behnken design under the response surface methodology. The optimum values of experimental parameters are found to be pH- 4.7; Temperature - 36.5oC; Fermentation time - 66.6hr; Inoculum concentration - 5.7% and Substrate concentration - 18.9 g/L. At these optimized conditions maximum amylase activity is found to be 16.89 U/ml.