Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats

The use of hematological leukocytes ratio indices (HLRI), which show the interrelations between different blood cells, for evaluation the organism state of laboratory rats is questionable. The aim is to investigate the informativity of HLRI in laboratory rats on the model of acute infectious process. The study performed on white nonlinear (7–8 months) male laboratory rats: control (n = 10) and experimental groups (n = 10), which simulated acute purulent fecal peritonitis. Analyzed the number of leukocytes, blood leukocyte formula, phagocytic activity of neutrophils, HLRI. Acute infectious process is accompanied by leukocytosis, neutrophilia with leukocyte blood formula shift to the left and inhibition of neutrophils absorption capacity. A set of changes of HLRI in experimental group animals reflects the predominance of cellular immunity over the humoral, prevalence of granulocytes and their immature forms, increased activity of the inflammatory process, predominance of the microphage system, intense non-specific immunity, disturbance of immune system functional state with a shift of balance towards monokines, formation of mainly delayed-type hypersensitivity reactions, decreased function of affector cells and predominance of effector part of immunological process, the infectious nature of intoxication. Analysis with receiver operating characteristic (ROC) curves displayed that 8 from 16 applied HLRI, such as blood leukocyte shift index, neutrophil to lymphocyte, segmented neutrophil to lymphocyte, banded neutrophil to lymphocyte, eosinophil to lymphocyte, monocyte to lymphocyte, neutrophil to monocyte and segmented neutrophil to monocyte count ratio indices have very high levels of sensitivity and specificity and can be used as diagnostic markers for acute purulent fecal peritonitis.