Effect of additives on short-term storage of ram semen

The aim of this study was to investigate the effects of short-term stored ram semen (24-hour intervals) on spermatological parameters up to 72 hours. In the study, % motility, viability and acrosome integrity were evaluated. In thıs study four ejaculates from each ram (four merino rams) were used. The ejaculates were divided into seven equal-sized pieces after pooling. The study groups were formed as resveratrol (2 and 4 mM), trolox (2 and 4 mM), BSA (3 and 6 mg/ml) and control added to tris-based diluent. Diluted samples were stored at 4 ° C until 72 hours. Spermatozoa motility, viability and acrosome integrity were evaluated at 0, 24, 48 and 72 hours. Motility examination was evaluated with phase contrast microscope at 400X magnification, vitality and acrosome integrity were evaluated with fluorescent microscope. At the 72nd hour of the study, the BSA (3 and 6mM) groups were assessed for motility (53.75 ± 2.50%; 55.00 ± 4.08%) and acrosome integrity (53.70 ± 3.39%; 57.10 ± 4.68%) in the control group (43.75 ± 2.50%; 46.53 ± 3.58%) and statistical difference (p <0.05). In the Trolox (4mM) group (67.22 ± 3.71%), the highest viability was achieved at 72 hours compared to all groups, and a statistical difference (p <0.05) was determined with the control group (46.53 ± 3.53). As a result of the study, it was determined that BSA and trolox added to ram semen extender were beneficial in the short-term storage of ram semen and had a protective effect on spermatological parameters.