Administration of Cocor Bebek Leaves Gel (Kalanchoe Pinnata) 20% Increased Neovaskularization, Number of Fibroblast Cells and Epitelization in the Wound Healing Process of Male Wistar Rats (Rattus Novergicus)

Background: Wounds are damage or deviation from normal anatomical and functional structures. Physiological changes that occur in the tissue regeneration process after injury are neovascularization, increased number of fibroblast cells and the occurrence of epithelialization. Cocor bebek leaves extract contains flavonoids, tannins, saponins and phenolic compounds that can accelerate the wound healing process. The purpose of this study was to prove that the gel of cocor bebek (Kalanchoe Pinnata) leaves extract could increase neovascularization, the number of fibroblasts, and epithelialization in the wound healing process of the skin of male Wistar strain mice (Rattus Norvegicus). Methods: This study used a randomized posttest only control group design using 28 male Wistar strain rats (Rattus Norvegicus) aged 4-6 months, 150-200 grams weight and healthy, divided into 4 groups, each group of 7 heads. After being injured, group P0 (4) was given base gel 2x / day for 4 days, group P1 (4) was given cocorebek leaves extract gel 2x / day for 4 days, group P0 (11) was given base gel 2x / day for 11 days, group P1 (11) was given cocor bebek leaves extract gel 2x / day for 11 days. All rats were given oral amoxilin 10 mg 3 times / day for 3 days. On days 4 and 11, skin samples were taken from the wound site for histopathological examination to see neovascularization, fibroblast cells, and epithelialization with Haematoxilin Eosin staining. Result: On day 4, the results showed that the mean number of neovascularization in the treatment group (4.60 ???? 3.37) was significantly higher than the control group (1.44 ???? 1.55) (p <0.05). There was a significant difference in the mean number of fibroblasts in the treatment group (45.71 ???? 9.95) which was higher than the control group (27.51 ???? 12.50). The mean epithelial length of the control group (85.71 ???? 28.35) and the treatment group (175.71 ???? 46.66) (p <0.05) were significantly different. On day 11, the results