Administration of Butterfly Pea (Clitoria ternatea) Flower Etanol Extract Oral Increased the Number of Pancreatic Beta Cells and Decrease the Level of Glycated Hemoglobin (HbA1C) of Diabetic Male Wistar Rats (Rattus norvegicus)

Type 2 Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia that occurs due to abnormalities in insulin secretion, insulin action or both. Chronic hyperglycemia causes a cumulation of glucose and hemoglobin bonds and an increase in the level of HbA1c. Experimental research was conducted with a randomized posttest only control group design. Subjects were while male Wistar rats (Rattus norvegicus). 36 animals treatment group were divided into two group (n=18 individuals). The control group was given aquabidest and glimepiride (0, 036mg/200gr body weight), and the treatment group was given butterfly pea flower part extract (80mg/200gr body weight) and glimepiride (0, 036mg/200gr body weight) via sonde once per day for 60 days. After 60 days of treatment, the immunohistochemical examination of pancreatic beta cells and HbA1c levels with the Rat Hemoglobin HbA1c Elisa kit. Analysis of pancreatic beta cells is the average number of pancreatic beta cells from all Langerhans islands in a three field preparation of 400x magnification by means of zigzag, the number of pancreatic beta cells is summed then averaged to get the mean number of pancreatic beta cells per large field of view. Result: There was a significant difference in the number of pancreatic beta cells in the treatment group 19.54????8.11 cells/field of view compared to the control group 8.84????5.32 cells/field of view (p<0.001). Meanwhile, in the HbA1c level there was a significant difference in in the HbA1c level in the treatment group 28.91????8.73 ng/mL when compared to the control group 35.78 ???? 8.96 ng/mL (p 0.013). Based on the results of this study it can be concluded that the combination of butterfly pea flower part extract and glimepiride increased the number of pancreatic beta cells and decreased the levels of HbA1c in diabetic male Wistar rats.