Detection of Pyogenic Infections among Clinical Samples Received in Department of Microbiology in Integral Institute of Medical Sciences and Research, Lucknow, Uttar Pradesh: A Retrospective Analysis

Background: Pyogenic infection is characterized by several local inflammation usually with formation of pus. A break in skin can provide entry to surface bacteria which thereby start multiplying locally. The body’s defense mechanism includes bringing immune cells into the area to fight against bacteria. Eventually, accumulation of these cells produces pus which is a thick whitish liquid. Objective of the study was to evaluate bacteria isolated from pus samples causing pyogenic infections and their antimicrobial susceptibility testing pattern. Material and Methods: A retrospective study was done over a period of 12 months from April 2018- March 2019 in the Department of Microbiology at Integral Institute of Medical Sciences and Research, Hospital Lucknow, India. The study was approved by the Ethical Research Committee (ERC) of the Institute. Data entry and statistical analysis were performed using the Microsoft Excel. Results: Total pus samples were 582, in which pathogenic bacteria were isolated in 429 samples (73.71 %) and number of bacterial isolates were 494 (84.87 %). In this study males were more affected 228 (53.14 %) than female 201 (46.85 %). Maximum number of patients belongs to age group 21-30 (20.74 %) followed by 11-20 (18.88 %). Conclusion: Gram positive bacteria S.aureus (43.72 %) is most common. E.coli (17.2 %) is the most common gram negative bacterial isolate followed by P.aeruginosa (13.56 %) which is second most common gram negative bacterial isolate. S.aureus was susceptible to linezolid (94.90 %), Vancomycin (98.61 %) and Teicoplanin (99.53 %).