Comparative Antimicrobial Efficacy of Chrysopogon Zizanioides and Matricaria Chamomilla along with 5.25% Sodium Hypochlorite and 2% Chlorhexidine against Enterocococcus Faecalis: An In-Vitro Study

Aim and Objective: The Aim of The Study Was To Compare the Antimicrobial Efficacy of Chrysopogon Zizanioides and Matricaria Chamomilla Along With 5.25 % Sodium Hypochlorite and 2 % Chlorhexidine against Enterocococcus Faecalis. Material and Method: In This Study, Essential Oils (100 % Pure Extract Free From Chemicals) Which Are Commercially Available Have Been Used. For media preparation, 15 g of agar powder were weighed and dissolved in 1000 mL by stirring in distilled water and sterilized by autoclaving at 15 lbs pressure (121°C) for 15 min. Total 16 agar plates were prepared by using nutrient agar. E. faecalis strain was inoculated and then streaked on nutrient agar plate to get single isolated colony. E faecalis were grown overnight at 37°C in nutrient agar. The antibacterial activity of: Vetiver essential oil [Group A], Chamomile essential oil [Group B], 5.25 % Sodium Hypochlorite [Group C] and, 2 % Chlorhexidine [Group D] were determined. Microbial zones of inhibition were measured in the number of colony forming units. Results: There was a significant reduction in the mean colony forming units in group C, which showed fluctuation from 41.75 % to only 6.75 % after the use of irrigant. The maximum percentage reduction was seen in group C being 83.83 % with minimum reduction in group A being only 22.67 %. Group B showed 58.95 % reduction which was comparable with Group D results showing 37.93 % reduction in the number of colonies Conclusion: The present study proves the efficacy of Chrysopogon Zizanioides and Matricaria Chamomilla against Enterococcus Faecalis which is comparable to 5.25 % Sodium Hypochlorite [I] and 2 % Chlorhexidine [III]. The Vertiver Essential oil [IV] proven to be the weaker irrigant than Chamomile Essential Oil [II] and 5.25 % sodium hypochlorite being most efficient amongst all as mentioned above.