Comparative Study on Hematological and Biochemical Parameters of Blood Units Stored in CPDA, CPD-SAMG and Leukoreduced Bags

Red cell units stored in the blood bank for the purpose of further transfusion.To ensure safety and therapeutic value, one need to follow instructions and standard operating procedures of a blood bank. Storage beyond stipulated temperatures could lead to storage lesions, these changes occur both in red cells and the suspending medium. These storage changes increase with the duration of storage of red cell units. This study aims at evaluation of in vitro effect of storage on hematological and biochemical parameters.OBJECTIVES: To determine changes in complete blood count, electrolytes (Na+, K+) and LDH changes in stored blood units on day 0, 1, 7, 14, 21 and 28. MATERIALS AND METHODS: Prospective study will be conducted in the blood bank, department of pathology and department of biochemistry, MAMC Agroha, Hisar. A total of 100 blood units were studied over a period of one year for storage lesions of blood. RESULTS: Prestorage leukoreduced red cell units showed significantly lower plasma potassium levels as compared to non-leukoreduced red cell units. It was observed that the fall in hematocrit with storage is least when blood is preserved in CPD-SAGM bags with Integral leukoreduction filters.It was also observed that rise in LDH level is least in Integral bag (indicates less hemolysis) as compared to single CPDA and triple CPD-SAGM bags. CONCLUSION: leukoreduction for specific indications can be undertaken to improve the quality of stored blood.