In Vitro Callus Induction studies in Salvadora persica L.

Present investigation has been focused on efficient in vitro callus induction protocol for Salvadora persica L. plant. Leaf, stem node and shoot tip explants from healthy plant were used as source of explant for callus induction. Maximum callus proliferation was obtained on Murashige and skoog medium supplemented with 2, 4 D. Callus induction differs according to nature of explant and concentration of growth hormone used. At the concentration of 1.0 mg/L of 2, 4 D massive callus was achieved using leaf as an explant whereas same result was achieved at the concentration of 1.5 mg/L and 2.5 mg/L concentration of 2, 4 D using stem node and shoot tip explant respectively. Three weeks old callus was used for shoot regeneration.