Ion Chromatography Method Development and Validation for Assay of Sodium Thiosulfate

A new ion chromatography method was developed, validated and adapted for the assay of Sodium Thiosulfate Pentahydrate as active pharmaceutical ingredient. In this method, separation of Thiosulfate was done using IonPac AS19 column with eluent of 45mM Potassium Hydroxide using suppressed conductivity detection. Thiosulfate was eluted at around 5.3 minutes. Linearity rangeis 1.0 ? 100.0mg/L with regression coefficient value of 0.9999 for Thiosulfate. The Limit of Detection (LOD) value found was 0.15mg/L, and the Limit of Quantification (LOQ) value of 0.06mg/L. Validation parameters examined following suggestions of ICH are accurate ample for the supposed assay. The approach is confirmed as splendid method for assay of Sodium Thiosulfate as active pharmaceutical ingredient with excellent assay percentage values.