The Antioxidant Activity Test of Rosella Flower (Hibiscus sabdariffa Linn.) with DPPH (1,1 -Dhenyl-2-picrylhydrazyl) and its Application for Lipstick Provisions

Rosella (Hibiscus sabdariffa Linn) grows a lot in the Java region and has never been analyzed and applied optimally in the health sector. The purpose of this study was to determine the antioxidant activity and application of anthocyanin dyes in lipstick preparations. This research using maceration and reflux extraction methods with ethanol fraction was carried out by Uv-Visible Spectrophotometry through DPPH reagents to obtain IC50 inhibitory concentration. The results of the test data were analyzed descriptively, and the extract was applied in the preparation of the lipstick preparations that were tested including organoleptic (physical, aroma, texture, and color), homogeneity test of lipstick preparations, test smear, melt temperature test, pH test, color intensity test, and irritation test The results of the research showed that the yield of rosella extract with maceration method was 88.72 g or 88.72% w/w, and for reflux method was 48.33 g or 48.24% w/w, the results of antioxidant test with IC50 value of extract rosella maceration is 11,940 ?g / mL while rosella reflux extract is 25,942 ?g / mL, this means that both extracts are very active as antioxidants (very high category), and the results of homogeneous lipstick formulation test, pH 4, melting point 50-700C, strength test 600-900 grams, and non-irritating.