Evaluation of Anti-Microbial (In-Vitro) Activity of Amarantha Herbal Mouthwash

Halitosis is one of the complaints encountered in the clinical settings where individual experiences unpleasant odours originating from oral cavity. Oral pathogens play important role in the generation of volatile sulphur compounds (VSC) in oral cavity that result into the development of bad breath. Mouthwashes are widely accepted to deliver antimicrobial agents so as to eliminate VSC producing bacteria and control bad breath. Most commonly used mouthwashes are effective but associated with multiple adverse effects like staining of teeth, gums, tongue and alteration of taste sensation after mouth rinsing. Ari Healthcare Pvt. Ltd. has conceptualized and developed Amarantha Herbal Mouthwash. The present study was conducted to evaluate antimicrobial activity of Amarantha Herbal Mouthwash against oral pathogens like Staphylococcus aureus, E. coli and Candida albicans. Muller Hinton agar was used for Staphylococcus aureus and E. coli and PDA was used for Candida albicans where in 100?l of cultures of respective microorganisms were spread. All experiments were performed in triplicate. The assessment of the antimicrobial activity was based on the measurement of the diameter of the zone of inhibition. The zone of inhibition for Amarantha Herbal Mouthwash against Staphylococcus aureus, E. coli and Candida albicans was 25mm, 17mm and 13mm respectively which suggests its significant antimicrobial activity against all the 3 microorganisms. This activity was comparable to that of standard Chlorhexidine. It can be concluded that Amarantha Herbal Mouthwash possesses antimicrobial activity against Staphylococcus aureus, E. coli and Candida albicans which further endorses its role in controlling Halitosis.