Assessments of Sero-Detection of Herpes Simplex virus-1 and 2 Antibodies IgG and IgM among Spontaneous Recurrent Miscarriage Women; Case Control Study in Gezira State 2018

Background: Recurrent miscarriage is a critical problem and it takes to increase during current decade, herpes simplex virus-1 and 2 have relation with recurrent miscarriage according to some previous study conducted in many countries. Objectives: theaim of present study to Sero-detection of herpes simplex virus -1 and 2 (IgG and IgM antibodies) by using ELISA techniques among women with recurrent miscarriage in Gezira state and assessment of other risk factors according to questionnaire. Method: Analytic - case control study (45 women in each arm) was conducted at Wad Madani teaching hospital Department of Obstetrics gynecological, AlGezira state, Sudan. The cases were women with recurrent miscarriage and controls were healthy pregnant women (non-miscarriage).Herpes simplex virus1and 2antibodies were analyzed in the sera of the entire participant using ELISA techniques. Results: Ninety women were enrolled in each arm of study. Miscarriage and non miscarriage serum IgG sero-positivity for HSV-1 44(97.1%) vs 41(91.1%) and HSV-2 is 13(28.9%) vs 4(8.9%) by ELISA. There was no significant difference in miscarriage serum IgM sero-positivity for HSV-1 16(35.6%) vs 6(13.3%) and borderline 2 (2.2%) vs 2 (4.4%) by ELISA. In logistic regression analysis of the predictors for miscarriage (OR=2.047, 95%Cl=.179-23.4.57, P.value 0.04).IgM sero-positivity were at risk for miscarriage.Other significant risk factors include microcytic hypochromic anemia, vaginal bleeding, pre-eclampsia and familyhistory. Conclusion: In the current study herpes simplex virus -1 and 2 IgG sero-positivity is associated with miscarriage. Using ELISA techniques are presumptive tools to confirm the results. Preventive measure should be implemented. Further research in needed. Keywords: HSV-1 and 2, antibodies, recurrent miscarriage women, ELISA.