In Vitro Anti-Hyaluronidase Activity Test of Alpiniazerumbet Rhizome Extract (Alpiniazerumbet (Pers.) B.L. Burttand R.M. Sm) and Preparation of Solid Lipid Nanoparticle

Alpinia zerumbet rhizome (Alpinia zerumbet (Pers.) BL Burtt and RM Sm) contains phenolic compounds that have anti-hyaluronidase activity that can reduce wrinkles on the skin. This study aims to test anti-hyaluronidase activity of Alpinia zerumbet rhizome extract using in vitro method and to prepare it in the form of nanoparticles with Solid Lipid nanoparticles (SLN) delivery system. Powder of Alpinia zerumbet rhizome was extracted with 70% ethanol using kinetic maceration method, then concentrated by rotary vacuum evaporator at 100 mmHg at a temperature of ±50°C. Anti-hyaluronidase activity of extract was tested using in vitro method with ELISA Reader, and then prepared into nanoparticles with SLN delivery system. Extract loaded SLN were prepared using polyethyleneglycol 8-beeswax and acrylyl glucoside under high speed homogenization-ultrasonication methods. Afterwards, extract loaded SLN was characterized. The test results of anti-hyaluronidase activity (IC50) of Alpinia zerumbet rhizome extract was 81.04 mg/mL. The characterization of Alpinia zerumbet rhizome extract loaded SLN were particle size, polydispersity index, zeta potential, and morphology analysis, all of the tests met the requirements of nanoparticle with results respectively, 138.6 nm; 0.226; -29.9 mV and a spherical shape. In conclusion, Alpinia zerumbet rhizome extract loaded SLN has potential as an anti-hyaluronidase agent with the ability to reduce wrinkles on the skin.