Molecular Characterization of Complement Receptor1 (Sl) and (McC) Polymorphisms in Patients with Black Grain Mycetoma, Gezira Mycetoma Center, Sudan (2019-2020)

The complement receptor1 (CR1) gene encodes for CR1protein as a component of the activity regulators. The Swain Langley (SL) and McCoy (McC) polymorphisms are common in CR1 and defined as the knob of blood group antigen. A Cross-sectional laboratory-based study was conducted to detect molecular characterization of complement receptor1 (SL) and (McC) Polymorphisms in black grain Mycetoma patients, Gezira Mycetoma Center, Sudan. Forty-one blood samples (3-5ml) were collected in EDTA tube. DNA was extracted using iNtRON's extraction kit. PCR reaction was done by using MyTaqTM Red Mix Kit. The polymorphisms for selected specimens (4) were determined by BGI Sanger sequencing. The participants were 28/41 (68.3%) males and 13/41 (31.7%) females consecutively. The age is ranging from (13-36) years. The PCR results revealed 34/41 (83%) were amplified whereas 7/41 (17%) were not. The sequencing of McC (SNP A4795G) shows a 50% is G allele and 50% an A-type. Whereas SL (SNPA4828G) bears 75 % G and 25% represents an A allele. In conclusion, the study supports the hypothesis of the SLallele and the McC allele and polymorphisms evolve in the Mycetoma predisposing factor. Further studies are recommended for screening and mapping the target sinuses surrounding the endemic and co-endemic areas.