Major Autohaemotherapy Reduced Free Radical Content and MDA of Heavy Smoker

The dangers of smoking on body health have been studied and proven by many researchers. High levels of free radicals in the blood of active smokers can trigger various diseases that are harmful to the body, such as emphysema and lung cancer. To avoid the diseases, the body requires intake of antioxidants from outside the body that can be obtained from food or vitamin supplementation. The therapy is a medical ozone therapy that uses ozone gas which is obtained from mixing pure oxygen and ozone gas. This study is an experimental study using a pre-test post-test control group design that is carried out on male smokers aged 30 to 50 years. The procedure of the study was subjects divided into 2 groups where the first group amounted to 8 people received ozone therapy 10 times with an interval of 1 week, the second group amounted to 8 people were controls who received placebo 1 cc injection of NaCl IV, as much as 10 times, with interval of 1 week. Blood free radical levels and MDA were examined in both groups at the beginning and end of the study with the FORM tool. The results showed a decrease in free radical levels in the blood in the group that was given major ozone. The results of the study using a comparison test after being given a treatment (post test) between the two groups with the t-independent test, found that the average level of free radicals in the blood with a FORM tool, the control group was 419.75±113.47, and the ozone therapy group 40 µg / ml was 311.00±39.17 (p less than0.05). The mean MDA level of the control group was 0.046±0.006, the mean of the ozone therapy group 40 µg / ml was 0.038±0.005. Significance analysis with the t-independent test showed that there were significant differences (p less than0.05). Based on the results of the study, showed a significant decrease in free radicals in the blood both measured by the value of FORT and MDA levels in the P1 group who were given ozone therapy 40 µg / ml for 10 weeks.