Studies on Degradation of Biofilm from Plant Extracts

Quorum sensing is a process of cell-cell communication that bacteria use to regulate collective behaviors. It depends on the production, detection and group-wise response to extracellular signal molecules called auto inducers. QS- Dependent regulation of gene expression controls wide variety of phenotypes including biofilm formation, drug resistant, virulence factors expression and motility. Administration of flavonoids to Pseudomonas aeruginosa alters transcription of quorum sensing controlled target promoters and suppresses virulence factor production, confirming their potential as anti-infective that do not function by traditional bactericidal or bacteriostatic mechanism. Various sources from plant i. e. Datura, Papaya, Merigold, Tea, Ganja, Capsicum and onion were collected and flavonoids were extracted from them using methanol extraction method. Biofilm was produced using MRS media and the extract was inoculated with the biofilm for 4-5 days. Dhatura, Papaya, Marigold, Tea, Ganja were able to degrade the biofilm. Effect of antibiotics were seen on degradation of biofilm, Gudchef and zefu have good tendency to degrade the biofilm. . Lactobacillus when inoculated along with the biofilm of P. aeruginosa, it inhibited the growth of the biofilm formation in P. aeruginosa