The Application of 5.4% Lime Peel Etanol Extract Cream (Citrus aurantifolia) inhibited the Increase of Matrix Metalloproteinase-1 Expression and the Decreased Amount of Collagen in Guinea Pigs (Cavia Porcellus) that were exposed to Ultraviolet B Rays

80 % of skin aging is caused by UV light exposure, which will increase the ROS production, one of which is characterized by an increase in Matrix Metalloproteinase-1 expression (MMP-1) and a decrease in the amount of collagen in the skin. Lime peel contains vitamin C, flavonoids, saponins, and tannins which prevent the formation of free radicals that inhibit the formation of excessive enzymes of Matrix Metalloproteinase. The purpose of this study is to prove that giving ethanol extract 5.4 % lime peel cream can inhibit the increase of Matrix metalloproteinase enzyme expression and decrease the amount of collagen in guinea pigs exposed to ultraviolet B. This study used a randomized posttest only control group design. The research subjects were guinea pigs (Cavia porcellus), totaling 36 guinea pigs which were divided into two groups, each with 18 individuals. The control group was given exposure to UV B rays and basic creams and the treatment group was given exposure to UVB rays and ethanol extract cream of lime skin 5.4 %. Total UVB radiation for 4 weeks was 840mJ / cm2, the treatment of base cream and lime peel extract cream of 5.4 % in each group was given every day, 20 minutes before and 4 hours after UVB radiation. MMP-1 enzyme expression was examined using the MMP-1 examination kit with immunohistochemical methods, while the amount of collagen was examined using histopathological methods with Pico Sirius Red staining. Then the data analysis was done based on the results obtained in the form of T independent test. The results showed the mean expression of MMP-1 in the treatment group was significantly lower than the control group (9.26±2.29 % vs 26.83±5.37 %; pless than0, 001). The mean collagen amount in the treatment group was higher than the control group (82.32±2.50 % vs 59.29±3.38 %; pless than0, 001). Based on the results of this study, it was concluded that application of 5.4 % lime peel extract cream inhibited the increase in MMP-1 expression and decreased the amount