High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC) for Qualitative and Quantitative Analysis of Chlorogenic Acid in Calendula Officinalis Linn

A simple Chromatographic technique such as HPTLC and HPLC provide valuable additional information to establish the identity of the plant material. The primary goal of this study was to develop a simple and reliable High Performance Liquid Chromatography and High Performance Thin Layer Chromatography method to quantitate polyphenol such as chlorogenic acid present in Calendula Officinalis Linn. using analytical techniques. A combination of this two analytical methods may be useful in quality control analysis as it would give a simple, precise and rapid method.