The Administration of Moringa Seed Oil (Moringaoleifera L.) Inhibited the Decrease of Leydig Cell and Testosterone Level in Male Wistar Rats (Rattusnorvegicus) that are Exposed to Cigarette Smokes

Moringaoleifera L. Kelor in Indonesian, the ripe seed is believed to have anti-aging properties. Cigarette smoke is one of the free radicals responsible for aging process. Decrease in testosterone is one of the indicators of the occurrence of aging process. Moringa seed oil (MSO) contains a number of chemical substances: alkaloid, phenol, flavonoid, sterol, tannin, saponin, protein, mineral, vitamin A, C, and E. This study was conducted to explore the effects of MSO on testosterone level and generate histology description of Leydig cell in Wistar rats. The study was a true experimental study that applied posttest-only controlled group as its research design. The samples in this study were 32 male healthy Wistar rats aged 6 months with body weight of 200-230 grams, which were divided into 2 groups, with 16 rats each. The control group was administered with plasebo (aquadest) with 1 ml/100 grams bw/day dosage, while the treatment group was administered with MSO with 2ml/kg bw/day dosage immediately after being exposed to 4 sticks of cloves (kretek) cigarettes. The treatment was given once a day for 14 days. On day 22, the rats were euthanized, then measured testosterone level with ELISA method and testicle surgery was conducted to generate microscopic supply, calculate the number of Leydig cells. The analysis result of the Leydig cells calculation showed that the average total /1 HPF in the control group was 20.33±4.19 and in the treatment group was 32, 41±7, 26 with p0.001. The average value of testosterone level in the controlled group was 2.237±0.401 and the treatment group was 2.938±0.326. With an independent sample t test, the number of Leydig cells in the treatment group was more significant (p0, 001) and testosterone level showed significantly higher in the treatment group (p0, 001). The concluded that administration of MSO potentially inhibits the decreased of Leydig cells and testosterone level in male Wistar rats that were exposed to cigarette s