The Effect of Some Plant Growth Regulators on Cell Biomass in the Cell Suspension Culture of Calendula Officinalis L. Calendula Arvensis L. Species
Journal: International Journal of Science and Research (IJSR) (Vol.8, No. 1)Publication Date: 2019-01-05
Authors : Nergis Kaya;
Page : 1719-1728
Keywords : Calendula officinalis; Calendula arvensis; cell suspension culture; plant growth regulators;
Abstract
The cell suspension culture C. officinalis and C. arvensis plants having medicinal and economical important was carried out in the four different MS medium which supplemented with different concentrations of NAA: BAP (1: 1, 0.5: 5 mg/l) and IAA: BAP (1: 1, 0.5: 5 mg/l) under sterile conditions. For this purpose, four months old calli which grown in the callus cultures were passed into the cell suspension culture. In this cell suspension cultures, cell counting and measurement of fresh/dry weight process were realized for two Calendula species during 40 days. This process repeated once in a five days. All of the research results were evaluated with Tukey Multiple Comparison Test.
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