Ethanol Extract of Annona Muricata L. Leaves Reduced Goblet Cell in Bronchus of Animal Model of Smoking

Dangerous effect of smoking like cancer, impotence, COPD and disorders of pregnancy and fetus are well known. COPD is well known as a diseases burden in developing country. Chronic inflammation in COPD leads to airways remodeling which is mark by Goblet cell hyperplasia and mucous overproduction. Annona muricata L leaves has been used for herbal for treating headache, diabetes, antiinflammation, dysnetry. It contains alkaloid and essential oil for antiinflammation. Based on our review there is no data about effect of Annona muricata L leaves with animal model of smoking. Twenty five mice were obtained from Animal Lab Facility, Faculty of Medicine, Udayana University. Mice with 8-10 weeks old and 25-30 gr weight were randomly chosen and adaptated for 7 days. Mice were divided into 5 groups and exposed with smoking by using small pump and container for 30 minutes once daily for 14 days. Group 1 (P1) was normal mice without smoking, grup 2 (P2) was mice with smoking, group 3 (P3) was mice with smoking and 25 mg/kg weight extract, group 4 (P4) was mice with smoking and 50 mg/kg weight extract, group 5 (P5) was mice with smoking and 75 mg/kg weight extract. After 14 days the mice was sacrificied. ANOVA analysis found significant difference in goblet cell number among the five groups (Figure 2) (p