Isolation of Autologous Stromal Vascular Fraction (SVF) from Adipose Tissue (Human Lipoaspirates): A Clinical Protocol

The autologous stromal vascular fraction (SVF) isolation from adipose tissue is an alternative to cultured adipose-derived stem cells in the regenerative medicine. However, the bioactivity of autologous SVF is not totally documented under disease conditions in patients. This clinical protocol aimed to describe an easy clinic protocol for SVF isolation from abdominal fat in healthy subjects subjected to liposuction / lipoaspiration; the protocol described all steps for aqueous and fat phases isolation by separation with collagenese (figures 1-3). Our original procedure for SVF isolation is useful for patients in the field of regenerative medicine; The autologous injection could prevent some pathogical conditions and also here illustrate the potential protective/reparative role of SVF in regenerative medicine field in patients.