Extraction of Crude Acid Protease from Goat (Capra aegagrus) Abomasum
Journal: International Journal of Science and Research (IJSR) (Vol.8, No. 5)Publication Date: 2019-05-05
Authors : Tanalyna Hasna; Sri Mulyani; Umar Santoso; Yudi Pranoto;
Page : 1721-1722
Keywords : ammonium sulphate; CAPGA; enzyme activity; extraction;
Abstract
The present study reports the extraction of crude acid protease from goat abomasum (CAPGA). The CAPGA was partially purified using different levels of ammonium sulphate precipitation and dialysis with dialysis sack MWCO 12 KDa. Protein concentration was determined by Bradford method using bovine serum albumin as the standard. The result shows that CAPGA extracted by ammonium sulphate precipitation of 75-100 % has highest protolithic activity. CAPGA activity and specific activity after being dialyzed was higher than before being dialyzed.
Other Latest Articles
- Unusual Presentation of a Rare Case of Nonketotic Hyperglycemia with Brain Parenchymal Findings
- A Tale of Candidal Infections from a Tertiary Care Centre
- Constructs Embedded in the National Achievement Test Results of the Philippines
- Academic Discriminants of Poverty
- Green Synthesis of Gold Nanoparticle using Nutmeg Fruit Extract
Last modified: 2021-06-28 18:12:38