ResearchBib Share Your Research, Maximize Your Social Impacts
Sign for Notice Everyday Sign up >> Login

Extraction of Crude Acid Protease from Goat (Capra aegagrus) Abomasum

Journal: International Journal of Science and Research (IJSR) (Vol.8, No. 5)

Publication Date:

Authors : ; ; ; ;

Page : 1721-1722

Keywords : ammonium sulphate; CAPGA; enzyme activity; extraction;

Source : Downloadexternal Find it from : Google Scholarexternal

Abstract

The present study reports the extraction of crude acid protease from goat abomasum (CAPGA). The CAPGA was partially purified using different levels of ammonium sulphate precipitation and dialysis with dialysis sack MWCO 12 KDa. Protein concentration was determined by Bradford method using bovine serum albumin as the standard. The result shows that CAPGA extracted by ammonium sulphate precipitation of 75-100 % has highest protolithic activity. CAPGA activity and specific activity after being dialyzed was higher than before being dialyzed.

Last modified: 2021-06-28 18:12:38