The Effectiveness of Faloak Extract on Superoxide Anion Radical (O2-) Activity in Kidney Organ of Tilapia Exposed to Mercury
Journal: International Journal of Science and Research (IJSR) (Vol.8, No. 6)Publication Date: 2019-06-05
Authors : Jannes Bastian Selly; Andreas Umbu Roga; Jacob M. Ratu;
Page : 1862-1864
Keywords : Antioxidant; Faloak extract; Mercury; Superoxide Anion radicals O2-;
Abstract
Research has been conducted to determine the radical content of superoxide anion (O2-) on kidney organs exposed to mercury before and after the administration of faloak (Stercullia quadrifida R. Br) extract as an antioxidant. The mercury concentration used in this study was 0.018 ppm. The activity of radical compounds was measured using Electron Spin Resonance (ESR) equipment, with the output of the Lissajous curve displayed on the oscilloscope. The extent of the curve illustrates radical activity, the wider the curve the more reactive radicals are. Faloak extract was varied in seven different concentrations, namely 0.25 mg/ml; 0.50 mg/ml; 1.00 mg/ml; 2.00 mg/ml; 4.00 mg/ml; 8.00 mg/ml and 16.0 mg/ml. After measurement, it was found that faloak extract was able to reduce O2- radical activity, starting from 1.00 mg/ml to 16.0 mg/ml. O2- radical activity was no longer identified after the administration of faloak extract at a concentration of 4.00 mg/ml; 8.00 mg/ml and 16.0 mg/ml.
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