The Value of Immunohistochemistry of P53 and Ki67 in Non-Hodgkin's Lymphoma

Aims-To determine the frequency of P53& Ki67 proteins, as detected by immunohistochemistry in lymph node biopsies of Iraqi NHL patients, to investigate their pattern of expression and to study the correlations and comparisons between P53 and Ki67 immunohistochemical detections and various clinical and histological parameters of NHL patients in order to specify their importance as prognostic factors in NHL patients. A total of 85 non-Hodgkin's lymphoma Iraqi patients including 54 males and 31 females cases, who has lymph nodes biopsies. Using the Working Formulation classification, the most common grade encountered was the Intermediate grade NHL constituted (65.9%) of the cases, while the lowest commoner was the Low grade with frequency of (15.3%). High grade lymphoma was encountered in (18.8%). The overall frequency of both P53 and Ki67 proteins positivity in NHL cases included was (63.5%) and (68.2%) respectively. Regarding the P53 and Ki67 results, no significant difference were found concerning age, sex, site of lymph node biopsy, and sclerosis which present in some morphological subtype of NHL. However there were statistically significant differences between P53 or Ki67 and different histological grade of NHL, whereby both P53 positivity and Ki67 expression were found to increase significantly (P