Comparative Analysis on GCMS, Physicochemical and Anti-Microbial Properties of Aerial Parts of Plant Artemisia vulgaris Obtained from Two Different Altitudes

The oil obtained by hydro distillation of Artemisia vulgaris using Clevenger apparatus revealed presence of alkanes, oxygenated and non-oxygenated monoterpenes, and sesquiterpenes. The oxygenated monoterpenes were either alcohols or esters, and the oxygenated sesquiterpenes were in the form of acetates and oxides. AVO1 contained 70 different constituents whereas AVO2 contained about 42 different chemical constituents. The physiochemical characters showed that the yield percentage varied from 1.43 to 1.28, acid value varied from 0.397 to 0.45and specific gravity varied from 0.878 to 0.902 in AVO1 and AVO2 respectively. Likewise the oil was found to be insoluble in water and fixed oil and soluble in most organic compounds. Volatile oil from both the altitude showed a significant antibacterial and antifungal effect. A large inhibition was seen in S. aureus, E. coli and enterococci species. Both the sample showed antifungal activity against Candida species.