Antibodies Production Comparison against SRBC between Normal and Probiotic Treated Chicks
Journal: International Journal of Science and Research (IJSR) (Vol.8, No. 12)Publication Date: 2019-12-05
Authors : Shaik Haseenabi; Kondapalli Kasturi;
Page : 329-333
Keywords : Hemagglutination inhibition test; SRBC; Probiotics; Antibody;
Abstract
The present study was stated that the antibody production comparison against sheep red blood cells between probiotic treated and normal feed treated chicks. The total of 3 batches were used for this experiment. First batch chicks were fed with normal feed treated as control and fed and 2ndgroup was treated with probiotics and feed diet and 3rd group was treated with normal diet but inoculation sample was formaldehyde-treated sheep red blood cells. when blood samples were taken as week intervals and go for the hemagglutination inhibition assay. The results were observed at weekly intervals, and the last week the antibody titer was observed highest in probiotic treated group shows the highest dilution at 1: 5120. Where as normal feed treated group contains dilutions of 1: 1280 higher than the Inactivated sheep red blood cells inoculated group of 1: 640. Inoculated sample quantity was increased as weeks increased as 0.5ml to 2ml at final week. The final large quantity of samples were stored in conical flasks for future purpose.
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