Comparative Study of Some Antibiotic Resistance, Extended Spectrum ?-Lactamases (ESBLs), Metallo ?-Lactamases (MBLs) Produced in Pseudomonas aeruginosa Isolated from Clinical and Food Samples

A total number of 33 isolates of Pseudomoans aeruginosa were collected from different clinical samples, such as burn, wound and urine from patients attending Al-Yarmouk teaching hospital and some private clinical laboratories in Baghdad city through the period from October to December 2016. On the other hand, 21 isolates of P. aeruginosa were collected from 38 different food samples, such as vegetables and fruits, from different local markets in Baghdad city during the period from November to December 2016. All isolates were identified by using different bacteriological and biochemical assays and confirmed by Vitek-2 identification system. The antimicrobial susceptibility test for clinical and food isolates towards 17 antimicrobial agents disks was preformed, the results showed that all clinical isolates were sensitive to Tobramycin but resistant to Cefepime, cloxacillin and Azthromycin, while all food isolates were sensitive to Ciprofloxacin but resistant to Cefepime. The resistant of P. aeruginosa clinical isolates towards Cefotaxime, Ceftazidime and Imipenem was 75.8 %, 51.5 % and 75.8 % respectively while the food isolates of P. aeruginosa showed 52.4 %, 95.2 % and 28.6 % resistant for same antimicrobial agents respectively. Double disk synergy method is used to detect of P. aeruginosa producing Extended spectrum -lactamases (ESBLs), 51.5 % of clinical isolates and 38 % of food isolates had the ability to produce ESBLs enzymes. Production of Metallo -lactamases (MBLs) was preformed by Imipenem EDTA combined disk method. The results reveal that 51.5 % of clinical isolates and 47.6 % of food isolates were MBLs producers.