Evaluation of Antibacterial Activity of Adhesive Systems Reinforced by Fluoroapatite or Calcium Fluoride (In Vitro Study)

Objectives The purpose of this study was to evaluate and test the antibacterial activity of ideal ratio addition of Fluoroapatite or calcium fluoride to the Total etch and Self etch adhesive systems and were compared to the control non-fluoridated adhesives against selected microorganisms which were Streptococcus mutans and Lactobacilli. Methods Total etch adhesive system (Tetric N-Bond) and Self etch adhesive system (Tetric N-Bond Self-Etch) were used with ratio addition of Fluoroapatite or calcium fluoride that didnt affect the shear bond strength of adhesive systems. Ten discs for each of six groups (TE, TE +FA 3 %, TE + CaF2 5 %, SE, SE + FA 1 % and SE + CaF2 7 %) were prepared, 5 discs of each group used for each microorganism were used to assess the antimicrobial effect of the fluoridated adhesives in vitro by measuring the inhibition zone by direct contact against selected microorganisms and comparing it with control groups. Data were statistically-analyzed using ANOVA, LSD and T-tests. Results The result showed that, there were a significant difference between Fluoridated and control groups in antibacterial activity at P0.01 level. Conclusions All Fluoridated adhesives have antibacterial effects against streptococcus mutans and Lactobacilli.