Study of Enzymatic Activity and Biofilm Formation of candida albicans in Oral Isolates Obtained from Group of Patients with Invasive Oral Candidiasis

Background Candidiasis is one of the most common disease of human caused by several species of Candida spp. , The yeast Candida is a normal habitant of the mucous membrane of oral cavity which switch to pathogenic microorganism in immunocompromised people. multiple virulence factors are contributing to enhance the infection in the host. Adherence to host tissues, switching from yeast to hyphae and exoenzymes secretion are important virulence factors of Candida albicans. These hydrolytic enzymes play important roles in pathogenicity of Candida infection. ObjectiveThe present study was conducted with an aim to determine in a comparative study phospholipase, proteinase, haemolysin, lipase activities and biofilm formation in oral Candidiasis isolated from diseased group and healthy group isolates and patients group before and after treatment with Nystatine 500000IU/ml. material and methods A total of 40 Candida albicans were isolated from oral cavity of patients with symptoms of oral candidiasis and from 12 healthy group. The specimens were identified by standard microbiological methods up to species level and were inspected for production of hydrolytic enzymes and biofilm formation. ResultsPhospholipase activity was strong in 8.3 % of healthy isolates and 25 % of patients group, 40 % of patients group isolates produced strong proteinase activity and 0 % in healthy group, haemolysin activity was strong in 37.5 % of patient group and 8, 3 % of healthy group, lipase activity was strong in 0 % of isolates of patients and healthy groups, 35.7 % of Candida albicans of patient group showed strong biofilm formation in comparison to healthy group 8.3 %. Conclusion Candida albicans showed more extracellular hydrolytic enzyme activity more biofilm formation in patients group than control group, Both the C. albicans in patients and control groups are capable of producing extracellular hydrolytic enzymes and biofilm formation but in filmentous form more than yest form.