To Study the Effect of Genistein&Conventional Anticancer Drug (Docetaxel) Combination on Breast Cancer

Cancer is a group of diseases characterized by uncontrolled growth and spread of abnormal cells. Breast cancer, lung cancer, prostate cancer and colon cancer are responsible for more than 50 percent of total deaths due to cancer. Many naturally obtained compounds (e. g. Genistein, diadzin etc. ) are closely linked to cancer, and many natural antitumor compounds are used to treat cancer. In the current work Genistein was extracted and isolated from soy plant (Glycine max. ) plant, percent yield of Genistein was found 1.0 % of crude extract and extract was standardized through TLC, UV- Visible spectrophotometry, FT-IR, NMR etc. Drug-Drug interaction studies were done with the help of FT-IR and DSC before testing them on breast cancer cell lines. The present work is an attempt to study the effect of Docetaxel in combination with Genistein on both estrogen receptor (ER) positive cell lines and ER negative cell lines. The study through Combination index values demonstrated that Genistein and Docetaxel show synergistic effect on ER positive cell lines MCF-7 over all effective dose ranges. But it showed antagonistic effect when tested on ER negative cell lines MDAMB-231 over all effective dose ranges.