Synthesis, Spectral Studies and Biological Evaluation of Hydrazone Derivative as Antimicrobial and Anticancer Agents

Hydrazone is a class of organic compounds formed by the fusion of carbonyl compounds with hydrazine. In the last few decades, the hydrazones have emerged as biologically important pharmacophores. The present work deals with the synthesis and evaluation of a new series of (2, 4-Dinitrophenyl) hydrazone derivatives as the hydrazones were synthesized by the reaction of various ketones with substituted hydrazine. The antimicrobial activity of the synthesized derivatives was carried out against Gram-negative bacteria Escherichia coli (MTCC 40) and Gram-positive bacteria Bacillus subtilis (MTCC 441) and Staphylococcus aureus (MTCC 87) by using Cup-plate agar diffusion method. Ciprofloxacin was used as standard drug for antibacterial activity. Six compounds 3a, 3d, 3e, 3f, 3g and 3j possessed good to moderate anti microbial activity. Compound 3f and 3g exhibited good antimicrobial activity against all the strains. The derivatives 3a-3j were further screened for anticancer activity against MCF-7 cell line using Adriamycin as standard. Seven compound 3a, 3d, 3e, 3f, 3g, 3i, 3j possessed good to moderate anticancer activity. Compound 3f exhibited best results in both assay. The structures of the synthesized compounds were established by IR and NMR spectral studies.