Effect of Application of Silver Nanoparticle on Fibrin Density Post Dental Extraction (Trial at Sprague dawley Rats)

Post extraction bleeding is one of the most common in oral surgery procedures. It has been attributed to various factors that can be broadly classified as local and systemic factors. One of the management bleeding complications is non-surgical interventions included haemostatic agents application. Silver nanoparticle has been has been widely used in medicine, for example as a burn dressing, contraceptives, layers of surgical instruments and basic ingredients of bone prostheses and as anti-microbes. This study aims to analyze the effect of Silver Nanoparticle (SNP) on the hemostasis process after tooth extraction of Sprague dawley rats as test animals This study was a pure experimental study conducted on 27 animals of Sprague dawley rats after tooth extraction, which was divided into three groups of equal size. The first group was the control group, the second group was the treatment group that applied 250 ppm SNP gel, and the third group was the group that applied 500 ppm SNP gel. Tooth extraction and application of test materials on animal tooth sockets are carried out in anesthetized animals. After 5 minutes of application of the test material, the animal was euthanasized and necropted to take socket tissue samples for histopathological preparations and stained with Hematoxylin-Eosin. Assessment of the effectiveness of the test material was carried out based on assessment of fibrin density on histopathological preparations. The results showed the effect of SNP on the hemostasis process seen from fibrin density. This was demonstrated in the non-parametric Kruskal Wallis statistical test with Mann Whitney further test, fibrin density in 500 ppm SNP gel administration group described significant results. The conclusion of this study is that SNP gel can increase fibrin density after tooth extraction in Sprague dawley rats.