Effectiveness of Sambote Extract on Decreasing Blood Glucose Levels of Male White Rats (Rattus norvegicus) Induced with Sucrose

The need for very expensive heart drugs has encouraged researchers and the pharmaceutical industry to look for new breakthroughs in hopes of getting new heart drug compounds. The general objective of this study is to obtain new heart drug compounds by utilizing traditional medicinal plants so that sudden death rates can be suppressed. The specific aim is to obtain scientific information about the activities of diabetes plants Sambote or Pasote. This research will empower the potential of the biodiversity of medicinal plants used by the people in Minahasa tribe, North Sulawesi Indonesia by testing the ethanol extract of the plant Sambote. The research was carried out in stages starting from the introduction and identification of Sambote biologically and then followed by extracting the leaves of Sambote which were dried with ethanol. The extract is varied to determine the exact effect of reducing glucose levels. Then extract isappliedbysqueezing the extract through the mouth of the white male rat to analyze the antidiabetic effects of the Sambote extract. The two controls were used, namely positive control and negative control. Measurement of blood glucose levels or antidiabetic is used by the Autocheck Multi-monitoring System. The results obtained were that the measurement of the body weight of the initial 24 rats was around 120 to 220 g with an average of 194 g. The initial blood glucose content of rats is from 65 - 128 mg/dL with an average of 82.5 mg/dL. After statistical data processing that the average body weight of white male rats before treatment or initial control was 194.05 g and blood glucose content was 82.5 mg/dL. After being given sucrose treatment for 48 hours, it weighs around 160-220 g with an average of 184.41 g. Body weight decreased by 4.97 %. Then blood glucose levels around 68-228 mg/dL or an average of 76.27 mg/dL. Blood glucose levels increased by around 38.49 % after 48 hours of sucrose treatment. After the treatment of the Sambote extract decreased blood glucose levels from an average of 94.33 mg/dL to 88.67 mg/dL or decreased by 5.66 mg/dL after 48 hours of application. Ethanol extract of Sambote (Dizphania ambrosioides) can reduce blood glucose levels in white male rats.